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KMID : 0613820110210020227
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Journal of Life Science
2011 Volume.21 No. 2 p.227 ~ p.234
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Plant Cell Contact-Dependent Virulence Regulation of hrp Genes in Pseudomonas syringae pv. tabaci 11528
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Lee Jun-Seung
Cha Ji-Young
Baik Hyung-Suk
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Abstract
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The hrp gene cluster in the plant pathogen Pseudomonas syringae is a key determinant of pathogenicity. Recent studies have demonstrated that specific host cell induction of the Ralstonia solanacearum hrp gene cluster is controlled by the PrhA (plant regulator of hrp) receptor. To characterize the role that P. syringae PrhA plays in the virulence of plant cells, a prhA homolog was isolated from P. syringae pv. tabaci and a prhA mutant was constructed by allelic exchange. The prhA mutant had reduced virulence in the host plant, and co-culture of P. syringae pv. tabaci and plant cell suspensions induced a much higher level of hrpA gene transcription than culture in hrp-inducing minimal medium. These results indicate that PrhA of P. syringae is a putative pathogen-plant cell contact sensor, therefore, we used a hrpA-gfp reporter fusion to monitor the in situ expression of PrhA. The results of this study demonstrated that PrhA induces hrp gene expression in P. syringae pv. tabaci in the presence of plant cells.
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KEYWORD
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Plant regulator of hrp (PrhA), plant-pathogen interaction, Pseudomonas syringae pv. Tabaci, TonB-dependent siderophore receptor
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